determination of lethal dose of plant extract

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determination of lethal dose of plant extract

determination of lethal dose of plant extract

determination of lethal dose of plant extract

Determination of Lethal Doses of Volatile and Fixed Oils of Determination of lethal doses of several plants Lethal doses (mL/kg) Plant extract LD1 LD10 LD50 LD The lethal dose

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Determining LD50 for a plant extract researchgate.net

The arithmetic method of Karber [*] was used for the determination of LD50. n = total number of animal in a group. a = the difference between two successive doses of administered extract

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DETERMINATION OF MEDIAN LETHAL DOSE OF

the extract respectively and observed for one hour and ten minutes after every two hours for twenty four hours. Result: No mortality or any physical signs of toxicity were observed in any of the

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DETERMINATION OF LETHAL CONCENTRATION FIFTY

The Objective: The objective of the present study is to determine acute toxicity that is lethal concentration fifty (LC 50) of whole plant ethanolic extract of Amaranthus viridis (A1), Aerva sanguinolenta (A2) & Cynodon dactylon (A3) on Zebrafish (Danio rerio) embryos at a different time interval.

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EVALUATION OF THE LETHAL DOSE OF THE METHANOL

indiscriminate use of plant medicine without prejudice to the toxic effects of plant extracts of which the leaf of mangrove is one, the present investigation aims to determine the mean lethal dose (LD 50) of leaf extract of Rhizophora racemosa collected from Dodo River in

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Determination of median lethal dose of ethanolic

PDF On Dec 31, 2017, M. K. Yusuf and others published Determination of median lethal dose of ethanolic extract of Sesamum Indicum seeds in adult wistar rats Find, read and cite all the

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Lethal dose of fresh male flower extract of Cucurbita

2021-6-22  This study dealt on the Approximate Lethal Dose (ALD) and Median Lethal Dose (LD 50) determination of fresh male flower of squash plant. The flowers were macerated with 95% ethanol, dried and the extract was made into different test solutions.

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Acute Toxicity (Lethal Dose 50 Calculation) of Herbal Drug

2015-3-23  arithmetic method of Karber [15] was used for the determination of LD50. LD50 LD100 = −∑ ×(a bn) n = total number of animal in a group. a = the difference between two successive doses of administered extract/substance. b = the average number of dead animals in two successive doses. LD100 = Lethal dose causing the 100% death of all test animals.

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Determination of lethal dose (LD50) of Exotoxin A from

2013-10-15  Determination of lethal dose (LD50) of Exotoxin A from Pseudomonasaeruginosa26A in mice histopathology Essam F, Al-Jumaily1Bushra H. Saleh2 andShalal M. Hussain3 1Biotechnology Depart./ Institute of Genetic Engineering and Biotechnology for postgraduate studies, Baghdad University, IRAQ.

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Acute toxicity studies and determination of median

Method: The toxicity of A. absinthium extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay.

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Lethal dose of fresh male flower extract of Cucurbita

2021-6-22  This study dealt on the Approximate Lethal Dose (ALD) and Median Lethal Dose (LD 50) determination of fresh male flower of squash plant. The flowers were macerated with 95% ethanol, dried and the extract was made into different test solutions.

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Determination of median lethal dose of ethanolic

The aim of this research was to determine the median lethal dose of sesamum indicum seeds in Wistar rats. Methods: Twelve Wistar rats within the ages of five to six weeks and of weights, ranging from (98-160) g of both sexes were used for the experiment. They were categorized into two phases of I and II. Phase I consisted three (3) groups of three (3) animals each.

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EVALUATION OF THE LETHAL DOSE OF THE METHANOL

The largest dose that did not kill any rat was noted, as well as the smallest dose that killed all the animals. Acute Toxicity Screening This study was done to determine the median lethal dose (LD 50) of the methanol extract of Rhizophora racemosa. A total of thirty

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Acute Toxicity (Lethal Dose 50 Calculation) of Herbal

In this study, different doses (10, 100, 285, 500, 1000, 5000 and 10,000 mg/kg) of the extract was administered orally to the different groups of rats and mice. Signs of toxicity and possible death of animals were monitored for 24 hrs to calculate the median lethal dose (LD50) of somina. At the end of the study, all the animals in all the dose

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Determination of lethal dose (LD50) of Exotoxin A from

2013-10-15  Determination of lethal dose (LD50) of Exotoxin A from Pseudomonasaeruginosa26A in mice histopathology Essam F, Al-Jumaily1Bushra H. Saleh2 andShalal M. Hussain3 1Biotechnology Depart./ Institute of Genetic Engineering and Biotechnology for postgraduate studies, Baghdad University, IRAQ.

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Acute and Subacute Toxic Study of Aqueous Leaf Extract

Determination of lethal dose (LD 50) The mortality rate of the animals for each dose was computed [13,14]. The arithmetical method of Karber was used for the determination of LD 50 [15]. The interval mean of the dead animals in each group of animals was used as well as the difference between doses for the same interval. The product of

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A PILOT STUDY ON THE DETERMINATION OF

2020-12-29  A PILOT STUDY ON THE DETERMINATION OF ANTIOXIDANT POTENTIAL AND LETHAL The reducing power of the plant extract was determined by the method of Oyaizu et al[18] Varying concentrations of the extracts (20-100 µg) were mixed with 2.5 ml of phosphate buffer (0.2 M, pH = Least lethal dose in a group ∑ (axb) / N, Where N is the number

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Evaluation of Acute Toxicity of the Methanolic Extract

Safety of polyherbal extract is evaluated principally by estimation of acute oral toxicity. Within the study, even the highest dose of plant extract i.e. 4000 mg/kg did not show any symptoms of toxication and death in for animals. Therefore, this polyherbal extract even at 4000 mg/kg could also be recommended as safe and offers no harm to the

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(PDF) ACUTE TOXICITY (LETHAL DOSE 50

Calculation of Median Lethal dose (LD50)For each mice the observation were made for 24 hr and symptoms of toxicity and rate of mortality in each group were noted. At the end of study period expired animals were counted for the calculation of LD50. The arithmetic method of Karber (1931) was used for the determination of LD 50.

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Determination of LD50 and ED50 by dose response

2018-12-25  The starting of lethal effect was recorded at the dose of 300 mg/kg and the calculated LD 50 value was found 229.2 mg/kg dose. The toxic sign and symptoms after oral dose introduction in mice were convulsion and tremor. Dose dependent toxic effect in behaviour of mice included convulsion, tremor, unsteady gait, and respiratory distress to death.

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Lethal dose of fresh male flower extract of Cucurbita

2021-6-22  This study dealt on the Approximate Lethal Dose (ALD) and Median Lethal Dose (LD 50) determination of fresh male flower of squash plant. The flowers were macerated with 95% ethanol, dried and the extract was made into different test solutions.

get price

Acute Toxicity (Lethal Dose 50 Calculation) of Herbal

In this study, different doses (10, 100, 285, 500, 1000, 5000 and 10,000 mg/kg) of the extract was administered orally to the different groups of rats and mice. Signs of toxicity and possible death of animals were monitored for 24 hrs to calculate the median lethal dose (LD50) of somina. At the end of the study, all the animals in all the dose

get price

Determination of lethal dose (LD50) of Exotoxin A from

2013-10-15  Determination of lethal dose (LD50) of Exotoxin A from Pseudomonasaeruginosa26A in mice histopathology Essam F, Al-Jumaily1Bushra H. Saleh2 andShalal M. Hussain3 1Biotechnology Depart./ Institute of Genetic Engineering and Biotechnology for postgraduate studies, Baghdad University, IRAQ.

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Determination of Lethal Dose for Gamma Rays Induced

2017-6-3  In conclusion, lethal dose for any mutagen is essential to generate highest practicable mutants with lowest damage to the plant. The LD50 dose based on survival percentage of the seedlings, after treatment with diverse doses of gamma rays for the butter bean cultivar kkl-1

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Sub-lethal assessment of aqueous dried leaf extract of

2018-7-25  Lethal dose (LD50) study A standard method was adopted for the determination of median lethal dose (LD 50) of extract.16 The aqueous dried leaf extract of C. roseus was given to various groups (Table 1A). The animals were then observed for behavioral changes and mortality for 24 hours. Sub-lethal

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Acute and Subacute Toxic Study of Aqueous Leaf Extract

Determination of lethal dose (LD 50) The mortality rate of the animals for each dose was computed [13,14]. The arithmetical method of Karber was used for the determination of LD 50 [15]. The interval mean of the dead animals in each group of animals was used as well as the difference between doses for the same interval. The product of

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Determination of lethal concentration (LC50) values of

35, Kuçukgul Gulec et al.,Determination of Lethal Concentration (LC 50) Values of Cinnamomum zeylanicum _____ Introduction In Turkey, Cyprinids are the richest and the most important family of fish, and its members are distributed world-wide.

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Evaluation of Acute Toxicity of the Methanolic Extract

Safety of polyherbal extract is evaluated principally by estimation of acute oral toxicity. Within the study, even the highest dose of plant extract i.e. 4000 mg/kg did not show any symptoms of toxication and death in for animals. Therefore, this polyherbal extract even at 4000 mg/kg could also be recommended as safe and offers no harm to the

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In Vitro activity of Desmostachya bipinnata (L.) Stapf

2018-5-1  The dried plant extract was freshly suspended in distilled water just before administration. 2.4.2. Determination of median lethal dose (LD 50) LD 50 of the ethanol extract was determined as described before (El-Meligy et al., 2017). For this purpose, 5 groups of 5 mature mail Swiss albino mice (23–25 g body weight) each were used.

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Determination of LD50 and ED50 by dose response

2018-12-25  The starting of lethal effect was recorded at the dose of 300 mg/kg and the calculated LD 50 value was found 229.2 mg/kg dose. The toxic sign and symptoms after oral dose introduction in mice were convulsion and tremor. Dose dependent toxic effect in behaviour of mice included convulsion, tremor, unsteady gait, and respiratory distress to death.

get price